Category: Biology

Please read the attached pdf for information on how to complete the assignment. It’s a biology research assignment. WRITE IT IN APA STYLE, and include citation and reference where needed.

i have done the pigment report project and i have uploaded my work in the files, 3 sections left which are Figure 3, T-test table and conclusion the lab report instructions guidline are in the file with the 2 pigments data (cherries and turmeric)

Critical Thinking Essay
Answer the following questions: Is connecting the dots in the human family tree important? Is it important that we know who our ancestors were and what they were like? Explain why, and provide specific examples to support your rationale.  
Use the content of chapter 32 from your textbook in reference to Human Evolution to help answer these questions. Two to four well-written paragraphs are required.
The assignment will be graded for content, grammar, punctuation, and proper English.
Feel free to contact me with any questions or for help before submitting your work.

Brief Report: For your assignment, you will briefly analyze each dataset and summarize major patterns in a 700-900 word written report. This report should provide the following for your supervisor:
A neatly labeled digital graph created in Google Sheets or Microsoft Excel of population sizes versus time. Population sizes will first need to be calculated as described above.
Description of population trends with reference to your created graph. Please include clear identification of what growth pattern is being observed (exponential, logistic, fluctuations) and one hypothesis to explain the patterns.
Assessment of lake usage by coyotes with reference to the eDNA data.
Summary of coyote diet with reference to the scat samples data.
Statement of trophic level(s) occupied by the coyote with reference to the scat sample data.
Your report should be typed and neatly organized into sections. Each bullet point (besides the graph) should be a summarized paragraph (100-200 words) with headings.
Links I was provided:
https://www.researchgate.net/profile/Torrey-Rodgers/publication/280310925_Drinking_water_as_a_source_of_environmental_DNA_for_the_detection_of_terrestrial_wildlife_species/links/55b1594208ae9289a084cf05/Drinking-water-as-a-source-of-environmental-DNA-for-the-detection-of-terrestrial-wildlife-species.pdf
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174897

Please read the pdfs attached it has everything stating that’s required to do the assignment. there are two parts for the assignment

Find the answer to the following questions in the uploaded document.
(1) What is mismatch repair and why would its functional status have the potential to
impact immune checkpoint blockade therapy? How does this relate to the concept of
MANAs?
(2) What approach do the authors used to address the question of whether mismatch
repair defective cancers are susceptible to PD-1 blockade? 
(3) How many patients were enrolled? Consulting the text and Supplementary Methods,
what were the criteria for enrollment? 
(4) Summarizing the data in Figure 1 and Table S1, what type of response to treatment
did the authors observe? 
(5) What hypotheses were tested in Figure 2A and Figure 2B, how they were tested and
what were the results? 
(6) What was the goal of the analysis in Figure 3?
(7) What are the implications of this study?

Chemistry of Life, list the properties of water, there attributes and their significance living organisms in our society.

Once you start you have 80 minutes. It opens in 12 hours and is due in 36 hours. Please check attachments and only if you’re sure that you can do, place bid.

Watch
the Power Point The Seedless Vascular Plants: Ferns and Their
Relatives. We will concentrate on the Ferns. Read pages 22 – 31 in
Chapter 2 in the study guide on Ferns. Then complete the assignment:
The Laboratory Examination of the Fern draft
THIS IS A VERY EASY ASSIGNMENT, I AM SENDING YOU ALL THE INFORMATION YOU NEED AS IT WILL BE LINKED IN THE ATTACHMENTS BELOW. A PERFECT SCORE IS NEEDED, SO PLEASE DO YOUR BEST ON THIS.

MethodologyStudy DesignThis study will utilize both in vitro and in vivo models to investigate the role of autophagy in cellular stress responses and neurodegenerative diseases.In Vitro StudiesCell Culture: Human neuroblastoma SH-SY5Y cells and primary neuronal cultures will be used.Induction of Stress: Cells will be exposed to various stress conditions, including oxidative stress (hydrogen peroxide), nutrient deprivation, and hypoxia.Autophagy Assays: Autophagy will be monitored using techniques such as LC3 immunoblotting, fluorescence microscopy of GFP-LC3, and quantification of autophagic flux using inhibitors like bafilomycin A1.Protein Aggregation: Assessment of protein aggregates (beta-amyloid, tau, alpha-synuclein, and mutant huntingtin) through immunocytochemistry and Western blotting.Cell Viability: MTT and LDH assays will be performed to assess cell viability under stress conditions.In Vivo StudiesAnimal Models: Transgenic mouse models of Alzheimer’s (APP/PS1), Parkinson’s (alpha-synuclein overexpression), and Huntington’s disease (R6/2 mice) will be used.Induction of Autophagy: Pharmacological agents (e.g., rapamycin) and genetic tools (e.g., ATG5/7 knockdown/knockout) will be employed to modulate autophagy.Behavioral Tests: Cognitive and motor functions will be evaluated using tests such as the Morris water maze, rotarod, and open field test.Histological Analysis: Brain sections will be examined for protein aggregates and neurodegeneration markers using immunohistochemistry and electron microscopy.Biochemical Assays: Brain tissues will be analyzed for autophagy markers (LC3, p62), inflammatory cytokines, and oxidative stress markers.Data AnalysisStatistical Analysis: Data will be analyzed using appropriate statistical methods (e.g., ANOVA, t-tests) to determine significance.Software Tools: ImageJ for image analysis, GraphPad Prism for statistical analysis, and other relevant bioinformatics tools for data interpretation.